Human serum albumin gradient in serous ovarian cancer cryosections measured by fluorescence lifetime
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چکیده
منابع مشابه
Interaction between alizarin and human serum albumin by fluorescence spectroscopy.
The binding properties on alizarin to human serum albumin (HSA) have been studied for the first time using fluorescence spectroscopy in combination with UV-visible absorbance spectroscopy. The results showed that alizarin strongly quenched the intrinsic fluorescence of HSA through a static quenching procedure, and non-radiation energy transfer occurred within the molecules. The number of bindin...
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Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...
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The binding of thyroxine by bovine and human serum albumin has been studied by measurements of the quenching of the ultraviolet fluorescence of the protein. The results obtained by this method have been compared with those of equilibrium dialysis measurements. Both approaches agree in assigning to serum albumin a single binding site of high affinity and three or more weak sites. The binding con...
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We present a novel fluorescence lifetime imaging microscopy technique to measure absolute positions of fluorescent molecules within 100 nm above a metalized surface based on distance-dependent fluorescence lifetime modulations. We apply this technique to fluorescently labeled microtubules as optical probes with various unlabeled proteins attached. By measuring the fluorescence lifetimes, we obt...
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The interaction of human serum albumin (HSA) with osthole was investigated by fluorescence spectroscopy. Osthole can quench the fluorescence of HSA and the quenching mechanism is a static process. The binding site number n and apparent binding constant K were measured at different temperatures. The thermodynamic parameters ΔH0, ΔG0 and ΔS0 were calculated at different temperatures. The results ...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2021
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.415456